Study of Chromogranin A in the cow; Physiological roles and practical application

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Iwate University
• Principal Investigator: Takahashi Toru 岩手大学, 農学部, 教授 (20355738)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ウシ / クロモグラニン A / 子宮

Outline of Final Research Achievements

This study was conducted on the expression dynamics and physiological role of bovine chromogranin A. We constructed a system for measuring the blood concentration of bovine chromogranin A and examined the blood dynamics in the early stages of pregnancy. In the conception cases, there were no major fluctuations and the values remained low, but in the non-conception cases, there were some that showed irregular fluctuations. The expression of chromogranin A in bovine uterine perfusate was examined by immunoblotting, and the relationship with the conception performance in the cycle immediately after collection of the perfusate was examined. As a result, increased expression was observed in non-conceived cases. When chromogranin A was added to bovine endometrial epithelial cells and changes in gene expression were examined, it was shown that chromogranin A promotes the expression of chemokines in the endometrium.

Free Research Field

家畜繁殖学

Academic Significance and Societal Importance of the Research Achievements

乳牛の受胎率は過去数十年にわたって低下の一途を辿っており、その改善が急務になっている。本研究では乳牛の受胎性に関与する可能性のある因子としてクロモグラニン Aというタンパク質に注目して、その発現動態と生理的役割について検討した。クロモグラニン Aの発現は受胎例と不受胎例で異なった血中動態を示し、子宮内におけるタンパク質発現量にも違いが認められた。