Epidemic survey of emerging avian pathogenic Escherichia coli and characteristic analysis using genomic information

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: University of Miyazaki
• Principal Investigator: Iguchi Atsushi 宮崎大学, 農学部, 准教授 (00437948)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 鶏 / 大腸菌

Outline of Final Research Achievements

Avian colibacillosis is a bacterial infectious disease that frequently occurs in broilers. The economic loss due to avian colibacillosis in Japan is large, and it is one of the matter of concerns to be improved in the field of agriculture and livestock. In this study, we performed isolation and detailed analysis of E. coli that may be caused by Avian colibacillosis at a poultry farm in Miyazaki Prefecture, which is the main production area of broilers. When the genome sequences of 276 strains derived from diseased chickens and 188 strains derived from healthy chickens were determined and analyzed in detail, one group that is phylogenetically very closely related, but strains show several different O-serogroups including O78, O115, and O161. From the above results, it became clear that a group of E. coli derived from the same origin and having different serotypes is prevalent in Japan.

Free Research Field

細菌学

Academic Significance and Societal Importance of the Research Achievements

これまで不明であった、国内で近年流行している鶏大腸菌症に起因する大腸菌の特徴が明らかとなった。病鶏から分離された大腸菌の血清型は多様であったが、ゲノム解析の結果、それらの一部は進化系統的に極めて近縁であった。つまり、同一起源から派生した、血清型が異なる大腸菌の一群が国内で流行している実態が明らかとなった。以上の結果は、対象菌を制御するためのワクチン開発などにとって、有用な情報になると考えられた。