2021 Fiscal Year Final Research Report
Do sperm preselect the sex of Japanese spiny rats (Genus Tokudaia) which lost their Y chromosome?
Project/Area Number |
19K06470
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 42040:Laboratory animal science-related
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Research Institution | University of Miyazaki |
Principal Investigator |
Koshimoto Chihiro 宮崎大学, フロンティア科学総合研究センター, 教授 (70295210)
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Co-Investigator(Kenkyū-buntansha) |
三谷 匡 近畿大学, 生物理工学部, 教授 (10322265)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 性染色体 / トゲネズミ属 / 減数分裂 / O-精子 / 実験動物管理 / 生殖工学技術 |
Outline of Final Research Achievements |
The spiny rat (genus Tokudaia), which has XO-type sex chromosomes without Y-chromosomes, inhabits only in Japan. Although this species is endangered and therefore difficult to utilize in fundamental research, we have attempted to analyze the sex chromosome composition of mature mammalian spermatozoa with XO-type chromosome configuration to provide evidence for a direct discussion of these meiotic mechanisms. However, as a preliminary step to approach rare samples, we have attempted to isolate sperm using FACS and segregate X- and Y-sperm by FISH in several model rodents, but without significant results in either case, we are continuing our research. On the other hand, we have obtained some secondary information that contributes to the reproductive engineering approach for wild-derived rodents.
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Free Research Field |
実験動物学
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Academic Significance and Societal Importance of the Research Achievements |
哺乳類の性を決定するY染色体の進化生物学的な意義を検討するため、XO型性染色体を有する日本固有種を対象としてその性決定機構の検討を試みたが、直接的な証拠を提示するための実験条件の設定に至ることができず、当初の主目的は達成できなかった。しかし本研究を進めるにあたって、絶滅危惧の希少種を対象として基礎研究を実施するために必要な野生由来の実験動物管理、または生殖工学に関する幾つかの基礎的知見が集積できた。今後はこれらの知見を活用して、当初の目的でXO-型を示す哺乳類における精子の性染色体構成比の解明と受精後の生殖細胞ゲノムの動態改正を継続していきたい。
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