Elucidation of a mechanism that protects lipid from oxidative stress in ciliated cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06560
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Shinohara Kyosuke 東京農工大学, 工学(系)研究科(研究院), 准教授 (20527387)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 繊毛 / 微小管 / 酸化ストレス

Outline of Final Research Achievements

In this project, we have analyzed unexpected phenotype of tracheal ciliated cells in a microtubule binding protein-deficient mouse (KO mouse). In the KO mouse, we observed lipofuscin in tracheal ciliated cells. Although we examined mechanism of this phenotype, any evidence was not obtained during the project. Alternatively, we have found another phenotype on the mucus secretory granule. In the KO mouse, ciliated cells harbored mucus secretory granule, suggesting that proportion of cilia-secretory hybrid cell increased compared with that in the wild type mouse. Further, we have validated this phenotype using immunofluorescence and RNAseq analysis.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究課題の成果により、当初の目的とは異なる方向性にはなったが哺乳類の気道繊毛細胞がどのような分子の働きで成熟するかについてその一端が明らかとなった。今回得られた知見を利用する事により、加齢や喫煙により呼吸器の繊毛細胞の数が減少し発症する病気の治療方法の開発に繋がる可能性がある。