Analysis of affinity changes of actin-binding proteins by mechanical response of actin molecular structures

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06603
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: Miyakonojo National College of Technology
• Principal Investigator: Noguchi Taro 都城工業高等専門学校, 物質工学科, 准教授 (90615866)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: アクチン / 力応答

Outline of Final Research Achievements

In this study, I investigated the changes in actin structure and the localization of the actin-binding proteins, cofilin and actinin, upon force stimulation of cells using FRETactin, which can detect the conformational state of actin.
As a result, I confirmed that the intracellular actin structure was changed by force stimulation. In addition, I found that force stimulation decreased the affinity of cofilin for actin, which is considered to be under tension, while actinin accumulated.

Free Research Field

生物物理

Academic Significance and Societal Importance of the Research Achievements

本研究により、細胞が力を受けると細胞内アクチンの分子構造やアクチン結合タンパク質の局在が変化することが確認された。細胞は生体内で血流や隣接する別の細胞、または細胞自身が持つ別のタンパク質は発生させる力など内外からの力によって、その機能を変化させることが知られている。
今回の成果は、その原理解明に重要な知見を与える可能性がある。