Molecular mechanism of nuclear structure construction by interaction between chromatin and nuclear pore complexes

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44010:Cell biology-related
• Research Institution: Osaka University
• Principal Investigator: Asakawa Haruhiko 大阪大学, 生命機能研究科, 准教授 (70399533)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 核膜 / 核膜孔 / 染色体 / クロマチン / 分裂酵母

Outline of Final Research Achievements

In interphase eukaryotic cells, chromosomes are stored in the nucleus, which is separated from the cytoplasm by the nuclear membrane, suggesting that chromosome function is regulated by nuclear membrane proteins. In this study, we analyzed the interaction between the nuclear pore complex and chromatin structure using fission yeast to elucidate the structure of the nuclear pore complex and to comprehensively identify proteins that interact with the nuclear pore complex proteins. This process revealed that the nuclear pore complex of fission yeast has a unique structure that is not found in any other species. We also identified several factors that genetically interact with the nuclear pore complex proteins.

Free Research Field

分子遺伝学、細胞生物学

Academic Significance and Societal Importance of the Research Achievements

核膜孔複合体タンパク質と密接な関連する可能性のあるタンパク質を多く同定することができた。今後の研究の進展によって、核膜孔複合体タンパク質の異常と関連の深い、様々な疾患との関係が明らかになれば、原因の解明や治療につながる可能性がある。学術的には、新規の核膜孔複合体の構造が明らかとなり、真核細胞の進化を理解する上で重要な成果を上げることができた。本研究は真核細胞の核膜の機能についてヒトを始め高等動物にも普遍的な分子メカニズムの理解や、疾患の原因の特定とその治療にも繋がると期待される。