2021 Fiscal Year Final Research Report
Study of amino acid sensing
| Project/Area Number |
19K06668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
Kamada Yoshiaki 基礎生物学研究所, 多様性生物学研究室, 助教 (20291891)
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| Co-Investigator(Kenkyū-buntansha) |
松浦 彰 千葉大学, 大学院理学研究院, 教授 (10272692)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 酵母 / アミノ酸 / シグナル伝達 / トア複合体 |
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| Outline of Final Research Achievements |
In this study I investigated the mechanism of Tor complex1 regulation by amino acid signaling. The goal of this study is to establish the novel model of TORC1 regulation by tRNA as an amino acid-starvation signal.
I found that tRNA binds to FRB domain of Tor1 protein. Since FRB domain is known as a rapamycin (a TORC1 specific inhibitor)- binding site, tRNA is supposed to regulate TORC1 activity in a same manner with rapamycin.
Therefore, I analyzed the tRNA-FRB binding using structural biological methods, and molecular biological methods to examine the above model.
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| Free Research Field |
分子遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
TORC1によるアミノ酸センシング研究において、Sabatiniが提唱する低分子量GTPase Ragを介したTORC1制御モデルが、多くの矛盾点を抱えつつも広く受け入れられている(Bar-Peled, 2013)。しかし、最近多くの疑問が国内外のTOR研究者により呈され、再考を余儀なくされている。本研究により提唱・検証された新規モデルは、TOR研究分野にパラダイムシフトを起こすことが期待される。
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