2021 Fiscal Year Final Research Report
Role of DNA methylation in phenotypic plasticities for environmental changes in plants
| Project/Area Number |
19K06719
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | エピジェネティクス / DNAメチル化 / エピ変異体 / シロイヌナズナ / 表現型可塑性 |
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| Outline of Final Research Achievements |
By phenotypic analyses and identification of causative genes of three kinds of epi-alleles, which are predicted to have caused phenotypes related to environmental responses and morphogenesis through changes in DNA methylation pattern, we obtained the following results. (1) A novel gene involved in the process of de-differentiation and regeneration was identified. This gene showed diverse methylation patterns among accessions. (2) In an epi-allele exhibiting pathogen resistance phenotype, expression of immune-related genes was increased only upon pathogen infection compared to the wild-type plant. (3) We identified two to three candidate causal genes of an epi-allele showing leaf morphological abnormalities related to auxin signaling on chromosomes 1 and 4.
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| Free Research Field |
植物分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
これまで報告されているエピ変異体は自然界に存在するものや偶発的に得られたものがほとんどであった。本研究によりエピ変異体のリソースとしてエピジェネティック組換え自殖系統群を用いることで、特定の表現型を示すエピ変異体の単離が可能であり、このようなエピ変異体では表現型が安定に遺伝することから、連鎖解析で原因遺伝子座を特定できることが示された。また病原菌抵抗性を示すエピ変異体では病原菌に感染した時のみ免疫関連遺伝子の発現が野生型より高く、従来の突然変異体や遺伝子組換え植物のように常に免疫機能を改変させ通常の生育にも影響を及ぼす植物とは異なる特性をもつ植物を得られた。
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