Optical recording and manipulation of cerebellum in fish navigation

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K06756
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
• Research Institution: Saitama University
• Principal Investigator: Tsuda Sachiko 埼玉大学, 理工学研究科, 准教授 (80736786)
• Co-Investigator(Kenkyū-buntansha): 平田 豊 中部大学, 工学部, 教授 (30329669)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 小脳 / ゼブラフィッシュ / カルシウムイメージング / 機能モジュール / 膜電位イメージング

Outline of Final Research Achievements

Spatiotemporal dynamics of Purkinje cell populations and its development were analyzed using zebrafish larvae by wide-field calcium imaging and quantitative analysis of neuronal populations. We visualized and characterized the spatiotemporal dynamics of clusters of relatively small Purkinje cell populations and the network structure they form. Furthermore, we experimentally demonstrated that this process could be activity dependent. We also established voltage imaging using ArcLight, a genetically encoded voltage indicator, in zebrafish larvae.

Free Research Field

神経発生学

Academic Significance and Societal Importance of the Research Achievements

本研究により、小脳の機能ネットワークの形成メカニズムの一端を、１細胞レベルかつ小脳広域を対象として明らかにできた。これは、ニューロン集団の秩序あるネットワークの出現機構そしてその情報処理機構の解明につながる成果であり、さらに小脳疾患などの理解への貢献が期待される。