単一細胞解析による覚醒制御機構の解明

2022 Fiscal Year Annual Research Report

• Project/Area Number: 19K06891
• Research Institution: University of Nagasaki
• Principal Investigator: 田中 進 長崎県立大学, 看護栄養学部, 教授 (30399472)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 網羅的遺伝子発現解析 / ナノポアシークエンス

Outline of Annual Research Achievements

今年度は、昨年度ナノポアシークエンス解析によって見出した処理群特異的な変化を示す遺伝子に関して機能解析を行った。患者から得られた組織並びに初代培養細胞での遺伝子発現変化を確認するとともに、組織切片上での発現の確認も行った。さらにその遺伝子を制御すると考えられる転写因子をsiRNAでKDし、遺伝子の発現の変化を確認した。樹立された細胞株を購入し、同様に処理群での発現変化を確認するともに、関連する薬剤を添加することでの変化も同定した。

Research Products

• [Journal Article] Degradation of STK16 via KCTD17 with ubiquitin-proteasome system in relation to sleep-wake cycle. (2023)
  • Author(s): Tanaka S*, Honda Y, Sawachika M, Futani K, Yoshida N, Kodama T.
  • Journal Title: Kinases Phosphatases Volume: 1 Pages: 14-22
  • DOI: 10.3390/kinasesphosphatases1010003
  • Peer Reviewed / Open Access
• [Journal Article] OLIG2 is an in vivo bookmarking transcription factor in the developing neural tube in mouse. (2022)
  • Author(s): Hayashi S, Oe S, Koike T, Seki-Omura R, Nakano Y, Hirahara Y, Tanaka S, Ito T, Yasukochi Y, Higasa K, Kitada M.
  • Journal Title: J Neurochem Volume: - Pages: -
  • DOI: 10.1111/jnc.15746.
  • Peer Reviewed
• [Journal Article] Establishment of neural stem cell culture from the central nervous system of the Iberian ribbed newt Pleurodeles waltl. (2022)
  • Author(s): Seki-Omura R, Hayashi S, Oe S, Koike T, Nakano Y, Hirahara Y, Tanaka S, Kitada M.
  • Journal Title: Dev Growth Differ Volume: 64 Pages: 494-500
  • DOI: 10.1111/dgd.12820.
  • Peer Reviewed / Open Access
• [Journal Article] 胎盤形成とnatural killer細胞 (2022)
  • Author(s): 村田 紘未, 田中 進, 岡田 英孝
  • Journal Title: 臨床免疫・アレルギー科 Volume: 78 Pages: 632-637
• [Journal Article] The regulators of human endometrial stromal cell decidualization. (2022)
  • Author(s): Murata H, Tanaka S*, Okada H*
  • Journal Title: Biomolecules Volume: 12 Pages: 1275
  • DOI: 10.3390/biom12091275
  • Peer Reviewed / Open Access
• [Journal Article] Sulfatide molecular species with ceramide composed of phytosphingosine (t18:0) and 2-hydroxy fatty acids in intercalated cells of the collecting duct in mouse kidneys. (2022)
  • Author(s): Nakashima K, Hirahara Y, Koike T, Tanaka S, Gamou K, Oe S, Hayashi S, Seki R, Nakano Y, Ohe C, Yoshida T, Kataoka Y, Tsuda M, Yamashita T, Honke K, Kitada M.
  • Journal Title: Lipid Res Volume: 63 Pages: 100210
  • DOI: 10.1016/j.jlr.2022.100210
  • Peer Reviewed
• [Journal Article] CPEB1 post-transcriptionally regulates Fmr1 expression through 3′UTR. (2022)
  • Author(s): Oe S, Hayashi S, Tanaka S, Koike T, Hirahara Y, Seki-Omura R, Kakizaki R, Sakamoto S, Nakano Y, Noda Y, Yamada H, Kitada M.
  • Journal Title: Front Cell Neurosci Volume: 16 Pages: 869398
  • DOI: 10.3389/fncel.2022.869398
  • Peer Reviewed / Open Access
• [Journal Article] A rare genetic variant in the cleavage site of the prepro-orexin gene is associated with idiopathic hypersomnia. (2022)
  • Author(s): Miyagawa T, Tanaka S, et al.
  • Journal Title: NPJ Genom Med Volume: 7 Pages: 29
  • DOI: 10.1038/s41525-022-00298-w.
  • Peer Reviewed / Open Access
• [Journal Article] Familial Hyperaldosteronism Type 3 with a Rapidly Growing Adrenal Tumor: An In Situ Aldosterone Imaging Study (2022)
  • Author(s): Takizawa N†, Tanaka S†, Nishimoto K*†, Sugiura Y, Suematsu M, Ohe C, Ohsugi H, Mizuno Y, Mukai K, Seki T, Oki K, Gomez-Sanchez CE, Matsuda T
  • Journal Title: Curr Issues Mol Biol Volume: 44 Pages: 128-138
  • DOI: 10.3390/cimb44010010.
  • Peer Reviewed / Open Access