2022 Fiscal Year Final Research Report
Single cell analysis to elucidate the regulatory mechanisms of arousal
| Project/Area Number |
19K06891
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | University of Nagasaki (2022)
Kansai Medical University (2019-2021) |
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Principal Investigator |
TANAKA SUSUMU 長崎県立大学, 看護栄養学部, 教授 (30399472)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 網羅的遺伝子発現解析 / ナノポアシークエンス |
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| Outline of Final Research Achievements |
The main focus of this study was the analysis using transgenic mice, however due to the effects of COVID19 and the restrictions of their breeding environment, it was difficult to carry out the analysis.
Therefore, I focused on the development of an analysis platform for nanopore sequencing, which is an analysis method, and adjusted minimap2 for mapping for long reads, mapped, counted reads with FeatureCounts, corrected, and extracted variable expression genes (FDR<0.01) with edgeR. This identified several splicing variant genes with altered mRNA copy number and Exon-Intron structure at each locus in the treatment groups in cultured cells used as a preliminary study.
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| Free Research Field |
分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
ナノポアシークエンスはRNAをRNAのまま配列決定することが可能なバイアスを抑えたシステムでありより生理的な状態の遺伝子発現変化を見るための優れた方法であるが、現在ナノポアシークエンス技術を用いたヒト組織を対象とする網羅的遺伝子発現解析は国内外を通じて29報しか報告されていない。これは解析パイプラインが整っていないこととコスト的な要因が大きいと考えられ、そのため我々は新規解析法を開発した。
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