Efficient separation method for nucleic acid aptamers using photochemical technique

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K07031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Sadakane Yutaka 鈴鹿医療科学大学, 薬学部, 教授 (60293304)
• Co-Investigator(Kenkyū-buntansha): 森本 正大 鈴鹿医療科学大学, 薬学部, 助教 (90824772)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 電気泳動 / 光反応基 / ジアジリン / S化DNA / トロンビン

Outline of Final Research Achievements

We developed a basic technology to enhance the production efficiency of nucleic acid aptamers, which are attracting attention as next generation antibody drug molecules. Our research achievement, the multi-analytical method, is a high resolution, rapid and simple analysis approach utilizing photochemical technology. In this study, we conducted the following steps to realize the analytical method: 1) establishment of a method for introducing photoreactive group into nucleic acid aptamers, 2) construction of a biding analysis method under denaturing conditions, and 3) evaluation of the multi-analytical method using model proteins. We successfully achieved simultaneous analysis of the binding specificity of two aptamers against thrombin in a single electrophoresis.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究成果である光反応基を用いた新技術は、１回の電気泳動で複数個の核酸アプタマーについて同時に多対象の結合特異性を解析できるという利点があります。核酸アプタマーのスクリーニングで得られる膨大な数の候補について、その結合特異性を調べることはとても骨の折れる大変な作業ですが、本研究の複数同時結合解析法を用いることで大幅に作業負担が軽減されると考えられます。核酸アプタマーの生産効率を高めることに役立つ基礎的な技術を開発できたことが本研究の意義と考えます。