Mast cell function is altered by Staphyloccocal superantigen-like protein 11

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07142
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 47050:Environmental and natural pharmaceutical resources-related
• Research Institution: Hoshi University
• Principal Investigator: Oku Teruaki 星薬科大学, 薬学部, 講師 (20409361)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: マスト細胞 / 黄色ブドウ球菌 / SSL11 / Coronin-1 / アレルギー / リン酸化 / PKCα / スーパー抗原様タンパク質

Outline of Final Research Achievements

First, we analyzed the degranulation of BMMC (mouse bone marrow derived mast cells) treated with recombinant SSL11. As a result, the mast cell treated with SSL11 reduced the release of beta-hexasominidase. Next, we attempted to isolate SSL11 binding proteins from BMMC by pull down assay. When the proteins bound by GST-SSL11 were analyzed by SDS-PAGE and silver staining, a major protein band of ~85 kDa was detected. This protein was analyzed by peptide mass fingerprinting with nanoLC-MS/MS. Furthermore, we have shown that coronin-1 is required for degranulation of mast cells (RBL-2H3) and phosphorylated at Ser412 by PKCalpha. These results will useful for the development of de novo allergy treatments.

Free Research Field

免疫学、生化学、分子生物学

Academic Significance and Societal Importance of the Research Achievements

日本国民の3人に1人が何かしらアレルギーを有しているといわれ“国民病”であるといえる。本研究成果は、外来物質（黄色ブドウ球菌産生物、Staphylococcal superantigen-like protein 11）および宿主因子（Coronin-1）の両者からマスト細胞の機能発現機構に注目し、アレルギー発症の分子機構を解明することで、新たなアレルギー制御法の開発に繋げるものである。また、黄色ブドウ球菌産生物質を利用しており、黄色ブドウ球菌のバイオリソースとしての可能性を探るものである。