2022 Fiscal Year Final Research Report
Mechanism of tissue-specific differentiation of vascular endothelial cells by Dach1 expression
| Project/Area Number |
19K07278
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 48010:Anatomy-related
|
|---|
| Research Institution | Kindai University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2023-03-31
|
| Keywords | 血管形成 / リンパ節 / 遺伝子発現マウス |
|---|
| Outline of Final Research Achievements |
In the present study, we analyzed the transgenic mice that ubiquitously express Dach1 gene. In lymph nodes, percentage of high-endothelial microvascular endothelial cells was increased in the Dach1 transgenic mice. In contrast, there was no change in the percentage of pan-endothelial marker-positive vascular endothelial cells. Next generation sequencing analysis showed that Dach1 transgenic mice showed differential expression of genes associated with high endothelial venules, suggesting that Dach1 regulates differentiation of lymph node vascular endothelial cells and vascular maturation.
|
| Free Research Field |
組織分化
|
| Academic Significance and Societal Importance of the Research Achievements |
癌組織およびリンパ節血管の解析から、転写因子Dach1 が組織特有の血管内皮細胞分化を決定する転写因子であることが明らかになった。次世代シークエンサー解析による血管内皮細胞の遺伝子発現パターンから、Dach1の被制御因子候補が得られ、Dach1によるリンパ節および腫瘍血管新生促進経路の一部が明らかになった。本研究の成果により、組織内微小環境下において血管多様性が生まれるメカニズムの一端が明らかになった。
|
