TN breast cancer histological analysis using the next generation PCR to create cancer gene panel testing

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07456
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Gunma University
• Principal Investigator: OYAMA TETSUNARI 群馬大学, 大学院医学系研究科, 教授 (50233622)
• Co-Investigator(Kenkyū-buntansha): 片山 彩香 群馬大学, 大学院医学系研究科, 助教 (60815695)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 乳癌 / トリプルネガティブ / 次世代PCR / パネル診断 / 病理組織

Outline of Final Research Achievements

The new molecular candidates targeting TNBC were selected from 23,609 genes using　transcriptome analysis in breast cancer cell lines, shRNA library targeting the TNBC cell lines and a public database of transcriptome analysis in normal tissues. The seven candidates selected were associated with TNBC viability and expressed highly in TNBC cells compared with their expression in non TNBC cells and in normal tissues. Using the selected genes, we tried to create new “panel” diagnosis methods of TNBC and histological characteristics of the new classified TNBC.

Free Research Field

人体病理学

Academic Significance and Societal Importance of the Research Achievements

TN乳癌は多様な種類の癌を含む一群で、ホルモン療法、分子標的療法抵抗性で、特に治療に関連した組織学的亜分類を見出すことが重要である。今回TN乳癌に関連する複数の遺伝子を見出し、臨床病理学的検討を加え、できればパネル診断まで発展できればと研究を進めた。今後の方針として、候補遺伝子の多重染色とAIを用いた解析が必要と考える。