Development of selective regulatory system for proliferation and persistence of CAR-T cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K07714
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 50020:Tumor diagnostics and therapeutics-related
• Research Institution: Jichi Medical University
• Principal Investigator: Uchibori Ryosuke 自治医科大学, 医学部, 講師 (20458285)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: キメラ抗原受容体 / CAR / 選択的制御遺伝子 / SRG

Outline of Final Research Achievements

In this study, we developed a selective regulatory gene (SRG) to selectively regulate proliferatoin and survival of CAR-T cells in vitro and in vivo. SRG-T cells were selectively expanded upon stimulation with an inducer (EPO). SRG-CAR-T cells showed cytolytic activity against CD19-positive target cells in vitro. In therapeutic experiments with tumor-bearing mice, SRG-CAR-T cells strongly suppressed tumor growth and all mice were survived even under conditions in which conventional CAR-T cells were unable to control tumor growth.

Free Research Field

免疫遺伝子細胞治療

Academic Significance and Societal Importance of the Research Achievements

本研究では、in vitroおよびin vivoで、CAR-T細胞の増殖・生存制御を選択的かつ効率よく可能にするSRG技術を開発した。この成果は、CAR-T療法の可能性拡大や有効性の向上に繋がる基盤技術として利用できることだけに留まらず、T細胞を用いたあらゆる免疫細胞療法に対しても広く波及することが期待される。