2021 Fiscal Year Final Research Report
Comprehensive research in morphogenesis of the cardiac outflow tract
Project/Area Number |
19K08308
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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Research Institution | Yamazaki University of Animal Nursing |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
栗原 裕基 東京大学, 大学院医学系研究科(医学部), 教授 (20221947)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 神経堤細胞 / 心臓 / 形態 / 弁 / 血管 |
Outline of Final Research Achievements |
The cardiac outflow tract is the region that connects the myocardium to the blood vessels, and abnormalities in this region are most frequent in congenital heart disease. The cells forming the cardiac outflow tract include second heart field derived cells, cardiac neural crest cells(postotic nccs), preotic nccs, intra-embryonic amniogenic somatopleural mesodermal cells, and epicardial progenitor cells. The purpose of this study is to construct a system to capture the morphogenetic process of the cardiac outflow tract during development in three dimensions and to elucidate the entire picture. We confirmed the distribution of nccs in the aortic and pulmonary valves in the neural crest marker Wnt-1-Cre mice. Furthermore, we found that the distribution of preotic and postotic nccs in the aortic and pulmonary valves differed in the chick chimara embryos.
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Free Research Field |
心臓発生学
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Academic Significance and Societal Importance of the Research Achievements |
先天性心疾患の発生原因、形態形成、治療および修復術の向上のためには発生過程における心臓形成の解析が有力な情報となる。先天性心臓疾患の中で最も異常が多く見られるのは心臓流出路である。心臓流出路は様々な由来部域から派生する細胞で構成され、二次心臓領域細胞、心臓神経堤細胞(後耳胞神経堤細胞)、前耳胞神経堤細胞、原羊膜壁側中胚葉、心外膜前駆細胞などある。本研究により後耳胞神経堤細胞が大動脈弁と肺動脈弁に分布するという事実は、弁の石灰化を考える上で重要な要素となり得、また発生の初期に出現する原羊膜壁側中胚葉が心臓に侵入し甲状腺の血管網にも関与していることは血管形成の研究分野に重要な情報を示すものである。
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