2021 Fiscal Year Final Research Report
Feedbak mechanism for host by microflora degradation of gastro-duodena gland mucin
| Project/Area Number |
19K08419
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 53010:Gastroenterology-related
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 胃腺粘液 / 糖鎖 / αGlcNAc / 腸内細菌叢 / マウス |
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| Outline of Final Research Achievements |
Whether the. gland mucin, which is abundant only in the stomach and duodenum, is degraded by intestinal bacteria and used as nutrition, and whether metabolites produced by intestinal bacteria are returned to the host in a transformed form. We attempted to elucidate this by examining differences in the intestinal microbiota of mice lacking αGlcNAc, a glycan structure characteristic of the gland mucin, and wild-type mice.
We found that intestinal bacteria possessing αGlcNAc-degrading enzymes were detected only in the cecum of wild-type mice. We also found that the αGlcNAc-dependent bacterial community, which has been reported to have glycoside hydrolase activity, was reduced in the cecum of the αGlcNAc-deficient mice compared to that of the wild-type mouse.
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| Free Research Field |
実験病理学
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| Academic Significance and Societal Importance of the Research Achievements |
消化管の内部を覆っている糖，蛋白質の複合体である粘液は，栄養源として利用可能で有り，分解する酵素を腸内細菌の一部が有している．腸内細菌叢は宿主にとって有用な成分を作っていることは広く知られ，この中に粘液に依存菌種も含まれている．食事・薬物の摂取などにより腸内細菌叢の構成菌種は影響を受けるものの代謝産物が共通することがわかっているが，ムチン依存菌であるAkkermansia muciniphilaは肥満や糖尿病との関連性が指摘されている．宿主の粘液成分の変化がムチン分解菌群の変化として宿主の健康に影響を与えている可能性が考えられ，すなわち粘液環境の改善を通して健康維持をはかれる可能性がある．
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