Mechanism of cancer stem cell formation induced by hepatocellular death

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08425
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Yokohama City University
• Principal Investigator: KONDO Masaaki 横浜市立大学, 医学研究科, 客員准教授 (60511615)
• Co-Investigator(Kenkyū-buntansha): 前田 愼 横浜市立大学, 医学研究科, 教授 (40415956)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 肝細胞癌 / 幹細胞 / 細胞死

Outline of Final Research Achievements

In this study, cancers caused by various cancer cell deaths are based on the hypothesis that the remaining cancer cells are transformed into malignant cells through stem cell formation or differentiation conversion due to cell death caused by anticancer agents, molecular target drugs, etc. We examined stem cell formation. A liver cancer cell line was subjected to cell death using 5-FU, Lenvatinib, etc., and an extract thereof was prepared. Cancer stem cell formation was monitored with the known cancer stem cell markers CD44 and E-cadherin. As a result, a slight increase in CD44 was observed when the -FU-treated extract was administered. When the mechanism was investigated, it was suggested that ASK1 / JNK activation was mediated by active oxygen (ROS).

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

治療によって惹起された細胞死がその悪性化に関連するのではないかと考えている。すなわち、そのメカニズムの解明、そしてその阻害療法の提言は肝細胞癌の悪性化を阻止し、その治療オプションとなることが期待される。