Search for corresponding antigens for a novel Crohn's disease-specific antibody

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08481
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Kurume University
• Principal Investigator: Mitsuyama Keiichi 久留米大学, 医学部, 教授 (20200066)
• Co-Investigator(Kenkyū-buntansha): 山崎 博 久留米大学, 医学部, 助教 (20529565) ; 吉岡 慎一郎 久留米大学, 医学部, 助教 (90425190)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ACP353 / TCP353 / クローン病 / バイオマーカー

Outline of Final Research Achievements

The main epitope of the ACP353-compatible antigen is the C-terminal 5 amino acid residue (GLFPN). In this study, we found multiple sequences that responded more strongly to Crohn's disease (CD) specimens than ACP353, and the feature on these sequences was CXXGLFPN. In other words, the reactivity of ACP353 could be improved by using Cys as the N-terminal of the antigenic peptide. These sequences are derived from human, bacteria, and parasite proteins, which have not yet determined as ACP353-compatible antigens. Furthermore, the reactivity of Escherichia coli AIEC and ACP353, which may be suspected as one of the causative bacteria of CD, was confirmed by the western blot method, but no specific one could be identified. Further studies are needed to determine ACP353-compatible antigens.

Free Research Field

消化管免疫

Academic Significance and Societal Importance of the Research Achievements

もしACP353の対応抗原が同定できれば、ACP353よりも優れた感度と特異度を持つクローン病(CD)診断マーカーの測定系を構築することができ、その結果CDの診断が内視鏡を使用せずに血清のみで行える可能性がある。
さらにACP353の対応抗原がCDの病因に関わっていることが明らかになれば、原因不明とされるCDの病態解明のbreakthroughとなり、ひいては根治療法へとつながる可能性がある。