In vivo imaging of DVT formation and organization to understand DVT pathogenesis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Kobe Pharmaceutical University (2020-2021); Kobe University (2019)
• Principal Investigator: Hara Tetsuya 神戸薬科大学, 薬学部, 准教授 (70547504)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 血栓

Outline of Final Research Achievements

DVT forms by Virchow's triad, which includes coagulation, endothelial cells, and blood flow. Therefore, it is difficult to understand using traditional cell culture, chemical, and pathological analyses. I have been trying to understand using bioimaging method. Recently, I established novel in vivo DVT imaging model. We newly visualized and clarified several new aspects of DVT formation and organization process in vivo.

Free Research Field

循環器内科

Academic Significance and Societal Importance of the Research Achievements

時空間軸で病態がダイナミックに変化する、血栓症のような循環器疾患の病態は、位置情報のない生化学的な蛋白解析や、時間軸の失われた病理サンプルでは理解が難しい。そのような中で、研究代表者が独自に開発した生体血栓モデルは既存の血栓モデルより実臨床のDVTに近い血栓を、生きたまま顕微鏡レベルで観察可能であり、DVTの理解が革新的に進むことが期待される。
血栓溶解療法は経験的に新鮮血栓であれば治療効果が高く、反対に器質化が進めば治療効果が望めない。この器質化機序を解明し、血栓溶解の効果が高い状態を維持できれば、DVTの治療戦略を大きく変えることができる。