Establishment of lung cancer and normal lung organoid library

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08610
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Keio University
• Principal Investigator: Hamamoto Junko 慶應義塾大学, 医学部(信濃町), 特任助教 (40570239)
• Co-Investigator(Kenkyū-buntansha): 安田 浩之 慶應義塾大学, 医学部(信濃町), 講師 (70365261)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 肺癌 / オルガノイド

Outline of Final Research Achievements

We established the lung cancer organoid library from more than 100 Japanese lung cancer patients. Airway organoid or alveolar type I organoid has been reported in 2015 or 2017 respectively however, culture method of alveolar type II has not been established until recently. We established the normal lung organoid library including airway and alveolar type II from same patient paired with lung cancer organoid first in the world. We identified molecular abnormalities from the analysis of multi-omics data of lung adenocarcinoma and small cell lung cancer organoids. We identified the possible therapeutic targets from the relation of molecular abnormality and phenotype difference.

Free Research Field

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Academic Significance and Societal Importance of the Research Achievements

オルガノイド樹立の試みは世界各国で行われ始めているが、我々は外科手術だけでなく様々な検査検体からもオルガノイド樹立を試みて成功しており、これらの技術は学術的独自性があるだけでなく、侵襲性の低い検体採取は患者への負担の軽減にもつながる。オルガノイドの特性として、癌部のみならず非癌部からも樹立できるという点から、正常細胞への毒性を考慮に入れた薬剤スクリーニング系の樹立へとつなげることも可能であり、創薬という観点からも大きなインパクトを与えることができると考えられる。