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2021 Fiscal Year Final Research Report

Establishment and analysis of humanized murine model for refractory asthma

Research Project

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Project/Area Number 19K08613
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53030:Respiratory medicine-related
Research InstitutionNihon University

Principal Investigator

GON Yasuhiro  日本大学, 医学部, 教授 (80339316)

Co-Investigator(Kenkyū-buntansha) 清水 哲男  日本大学, 医学部, 准教授 (00339326)
伊藤 亮治  公益財団法人実験動物中央研究所, 実験動物研究部, 室長 (60425436)
丸岡 秀一郎  日本大学, 医学部, 准教授 (80599358)
Project Period (FY) 2019-04-01 – 2022-03-31
Keywords気管支喘息 / IL-33 / TSLP / 好酸球 / 気道過敏性 / ヒト化マウス
Outline of Final Research Achievements

Human IL-3 / GM-CSF / IL-5 gene-introduced NOG mice (humanized mice) are established, and human IL-33 and TSLP are administered into the airway. An increase in eosinophils was observed in the airway. Administration of dexamethasone did not suppress human eosinophil infiltration by co-stimulation with IL-33 and TSLP. House dust allergen (Der-p2) -specific synthetic humanized IgE antibody was administered to the humanized mice for passive sensitization, and then the airway was stimulated with Der-p2. Infiltration of human eosinophils was confirmed, and an airway hypersensitivity test with methacholine confirmed an increase in airway hypersensitivity.

Free Research Field

呼吸器内科

Academic Significance and Societal Importance of the Research Achievements

世界で初めてヒト化喘息マウスモデルを樹立した。ヒト・サイトカインやヒト・炎症細胞によって,好酸球炎症や気道過敏性という喘息の特徴を保持しアレルギー炎症を惹起できる,アレルギー性喘息やステロイド抵抗性の難治性喘息のヒト化マウスモデルを作製することに成功した。本研究成果は,今後,ヒト化マウスモデルを用いて新たな治療薬や検査方法を開発するために有用な研究モデルとなることが期待される。

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Published: 2023-01-30  

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