Expansion of human iPS cell-derived ureteric bud organoids

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K08703
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Kyoto University
• Principal Investigator: Mae Shin-Ichi 京都大学, iPS細胞研究所, 特定拠点助教 (50749801)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: iPS細胞 / 尿管芽 / 腎臓 / 拡大培養

Outline of Final Research Achievements

We previously established the induction method for branching ureteric bud, one of the renal progenitors, from human induced pluripotent stem cells. In the current study, we have developed a novel expansion culture method for ureteric bud tip cells while maintaining the reconstitution ability of ureteric bud organoids. Moreover, we have clarified that NFkB pathway is crucial for the proliferation of ureteric bud tip cells and found that the long-term expansion culture advances the renal developmental stage to the late branching phase.

Free Research Field

再生医学

Academic Significance and Societal Importance of the Research Achievements

これまでのところ、尿管芽の分枝形態形成における組織幹細胞である尿管芽tip細胞を選択的に拡大培養し、大量の尿管芽オルガノイドを作製する方法は確立されていない。したがって、本研究の成果は新規性および進歩性に優れている。さらに、拡大培養によって発生分化段階が進んだ腎組織を作製可能になることで、腎臓発生機序の解明が進展し、iPS細胞を用いた先天性腎尿路異常の病態解明に貢献することが期待される。