2021 Fiscal Year Final Research Report

Development of functional muscle sheet using direct conversion technique and iBTA

Research Project

PDF

Project/Area Number	19K09053
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55010:General surgery and pediatric surgery-related
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Fumino Shigehisa 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40405254)
Co-Investigator(Kenkyū-buntansha)	松田修京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164) 岸田綱郎京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205) 坂井宏平京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (30515292) 田尻達郎京都府立医科大学, 医学(系)研究科(研究院), 教授 (80304806)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	骨格筋シート / バイオシート / ダイレクトリプログラミング
Outline of Final Research Achievements	In order to create skeletal muscle sheets for congenital skeletal muscle defects, we have developed a short time frame to create "functional" skeletal muscle sheets in a mouse abdominal wall defect model by using a direct programming method that efficiently transforms fibroblasts into multinuclear muscle cells and biosheets created by in vivo tissue formation technology. Specifically, multinucleated skeletal muscle cells differentiated from human fibroblasts by direct conversion were seeded onto a mouse abdominal wall defect model using a biosheet fabricated subcutaneously in mice as a scaffold, and transplanted into the mouse abdominal wall defect model for histological evaluation. The results showed some growth, but not enough. In vivo transplantation experiments using cross-linked nanogels will be performed in the future.
Free Research Field	小児腫瘍
Academic Significance and Societal Importance of the Research Achievements	本研究では，先天性骨格筋欠損に対しての骨格筋シートの作成を最終目標とする.線維芽細胞を高効率に多核筋細胞に形質転換するダイレクトリプログラミング法と生体内組織形成技術により作成したバイオシートを用いることで，マウス腹壁欠損モデルについて，自己細胞・組織から強度と運動性を担保した「機能する」骨格筋シートを短期間に作成し，実臨床において応用可能な治療プラットフォームの開発を目指す.