2021 Fiscal Year Final Research Report
Exploration of genome-wide molecular diagnostic markers in follicular carcinoma using thyroid cytology specimens.
| Project/Area Number |
19K09055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Nippon Medical School (2020-2021)
Osaka Prefecture University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石橋 宰 大阪府立大学, 生命環境科学研究科, 准教授 (70293214)
杉谷 巌 日本医科大学, 大学院医学研究科, 大学院教授 (50465936)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 甲状腺濾胞癌 / 甲状腺細胞診 / ゲノムワイド分子診断マーカー / RNA |
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| Outline of Final Research Achievements |
Genome-wide transcriptome analysis was performed on FFPE specimens to search for candidate biomarkers and drug targets. In addition, we performed real-time PCR expression analysis on follicular thyroid carcinoma(FTC) and follicular adenoma(FA) for the transcripts that we have identified as candidate markers. As a new finding, we found that a splice variant of mRNA encoding a chemokine-like protein is significantly upregulated in FTC tissues. This particular splice variant was shown to be specifically expressed in FTC, while it is rarely expressed in papillary thyroid carcinoma and FA. Therefore, this splice variant is expected to be clinically applicable as a FTC-specific biomarker.
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| Free Research Field |
内分泌外科
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、甲状腺濾胞癌から抽出したRNAを用い、臨床応用可能な癌死危険度診断マーカーとしての臨床応用への可能性を追求することを目的とした。その結果、新たな知見として約11 kDaのケモカイン様蛋白質をコードするmRNAのスプライスバリアントが濾胞癌組織において顕著に上昇することを明らかにした。本スプライスバリアントは、濾胞癌特異的バイオマーカーとしての臨床応用が期待されている。
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