2022 Fiscal Year Final Research Report

Novel therapeutic targets based on the interactional analaysis between fat cell and cancer cells in the patients with esophageal cancer

Research Project

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Project/Area Number	19K09231
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55020:Digestive surgery-related
Research Institution	Japanese Foundation for Cancer Research
Principal Investigator	WATANABE Masayuki 公益財団法人がん研究会, 有明病院消化器外科, 部長 (80254639)
Co-Investigator(Kenkyū-buntansha)	高松学公益財団法人がん研究会, 有明病院病理部, 医員 (00750366) 森誠一公益財団法人がん研究会, がんプレシジョン医療研究センター次世代がん研究シーズ育成プロジェクト, プロジェクトリーダー (10334814) 今村裕公益財団法人がん研究会, 有明病院消化器外科, 医長 (70583045) 井田智公益財団法人がん研究会, 有明病院消化器外科, 医長 (80583038)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	Obese / Esophaegal cancer / Complement factor / C3 / C4 / BMI
Outline of Final Research Achievements	We had already found that complement factor 3 (C3) and C4 was upregulating in omental fat tissue of obese patients with esophageal cancer (N=6). In this study, we aimed to validate this finding using larger sample size (N=152) and add gene expression analyses, and elucidate the underlying interaction between fat cells and cancer cells. After quantifying C3 and C4 expression levels by using multiple reference genes of POP4 and EIF2B1, C3 or C4 upregulation was not associated with obesity, in any of 13 obese classification based on BMI, visceral or subcutaneous fat area or grith of the abdomen at naval level on CT. Microarray analysis was not successful due to poor RNA quality. We did not observe any positive association between C3/C4 expression and obese in the patients with esophageal cancer.
Free Research Field	消化器外科
Academic Significance and Societal Importance of the Research Achievements	生活習慣と発がんや腫瘍進展に関して注目や興味が高まるものの、未だ明確なエビデンスは構築されていない。我々は過去のデータに基づき大網の補体活性化経路が重要なメカニズムであるのではないかと仮説を立て、特に本研究ではC3/C4遺伝子発現に着目し、152症例の大網脂肪サンプルを用いて検討したが、有意な相関関係は認められなかった。アーカイブされていた脂肪組織のRNAが分解が進み、解析に値するデータが得られなかったため、今後はフレッシュなサンプルを用いて肥満と癌の関連を解明予定である。