Research on functional evaluation and clinical application of mesenchymal stem cells derived from peri-arterial adipose tissue

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09237
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: University of Tsukuba
• Principal Investigator: Osaka Motoo 筑波大学, 医学医療系, 講師 (60816544)
• Co-Investigator(Kenkyū-buntansha): 大根田 修 筑波大学, 医学医療系, 教授 (30311872)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 間葉系幹細胞 / SDF-1 / CXCR7 / 創傷治癒 / 炎症反応

Outline of Final Research Achievements

In analysis of PVAT-MSCs in lesion site with arteriosclerosis, although the proliferation activity is low, the expression level of SDF-1/CXCR7 is high. These results suggested that PVAT-MSCs may be affect to disease progression. In order to analyze the cell function in vivo, PVAT-MSCs were analyzed using a mouse wound healing model, and it was found that the wound healing activity was increased. Since wound is repaired not only by direct tissue repair but also by various functions, we performed some in vivo assay such as expression analysis of inflammatory cytokines and migration assay of inflammatory cells and be able to find some results.

Free Research Field

心臓血管外科

Academic Significance and Societal Importance of the Research Achievements

人口構成の高齢化とともに動脈硬化性疾患もこれと平行して増加している。下肢アテローム硬化性閉塞性動脈疾患は、背景にある生活習慣病の治療による進行予防と、病変部位に対する血行再建（人工血管置換によるバイパス手術や血管拡張術）が行われている。いずれも対症療法であり、30-50％の患者に血行再建後の再閉塞を認め、下肢切断に至る例も少なくない。本研究課題の遂行により新規間葉系幹細胞を用いて効果的で安全な新しい治療法の開発に大きく貢献でき、QOLの改善など社会的意義も大きいと考えている。