Development of Biological Aoritic Stent Graft Based on Nobel Tissue Engineering

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09273
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 55030:Cardiovascular surgery-related
• Research Institution: Kumamoto University
• Principal Investigator: Okamoto Ken 熊本大学, 大学院生命科学研究部(医), 講師 (20372782)
• Co-Investigator(Kenkyū-buntansha): 田爪 宏和 熊本大学, 病院, 非常勤診療医師 (10648273) ; 福井 寿啓 熊本大学, 大学院生命科学研究部(医), 教授 (50445045) ; 野口 亮 熊本大学, 病院, 助教 (70530187)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 大動脈外科 / 組織工学 / 再生医療 / ステントグラフト

Outline of Final Research Achievements

In this study, we aimed to develop a new stentgraft that are resistant to infection.
we use the cells that make up blood vessels, such as human vascular endothelial cells, vascular smooth muscle cells, and fibroblasts, and use the cell aggregation phenomenon to construct vascular tissue-type spheroids in a three-dimensional manner to form blood vessels. Histological evaluation of extracellular matrix was performed. Compared to the native blood vessels, the production of these extracellular matrices tended to be irregularly distributed within the vessel wall. Endothelium cells were not distributed as endothelium to line the endometrium of the vascular structure, but were distributed inside the structure to form an immature vascular network. It was possible to develop a tissue engineering technique for constructing a three-dimensional vascular tissue only from cells, and to construct a three-dimensional vascular structure in three dimensions.

Free Research Field

心臓血管外科

Academic Significance and Societal Importance of the Research Achievements

本研究は細胞凝集現象を利用しながら、細胞のみで立体的な組織を作る技術を応用して
血管を構成する、血管内皮細胞、血管平滑筋細胞、線維芽細胞を大量培養し、血管組織型スフェロイド（細胞凝集塊）を作成さらに立体化させ血管組織型パッチを作成することを目的とした。その結果、分布形態は血管としては未熟であったが細胞のみを三次元化して立体血管構造体の構築を行うことが可能であり、感染に対して抵抗性を有するバイオロジカルなデバイス開発の可能性が示唆された。