2021 Fiscal Year Final Research Report

A novel diagnostic approach for chronic lung allograft dysfunction after lung transplantation

Research Project

PDF

Project/Area Number	19K09305
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 55040:Respiratory surgery-related
Research Institution	Okayama University
Principal Investigator	Sugimoto Seiichiro 岡山大学, 大学病院, 准教授 (40570148)
Co-Investigator(Kenkyū-buntansha)	豊岡伸一岡山大学, 医歯薬学域, 教授 (30397880) 大藤剛宏岡山大学, 大学病院, 教授 (40452578)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	肺移植 / 慢性移植肺機能不全（CLAD） / 慢性腎臓病（CKD） / バイオマーカー / 個別化医療 / マイクロRNA / 一塩基多型（SNP） / ジャポニカアレイ
Outline of Final Research Achievements	First, we investigated the role of circulating microRNAs in the diagnosis of chronic lung allograft dysfunction (CLAD) after bilateral lung transplantation (LT). Plasma levels of miR-21 were significantly higher in the CLAD group than in the non-CLAD group. The miR-21 levels were significantly correlated with the percent baseline value of the forced expiratory volume in 1 second, the forced vital capacity, and the total lung capacity at one year before and onset of CLAD. Circulating miR-21 were associated with the development of CLAD and appears to have the potential to detect CLAD after bilateral LT. Next, we investigated single-nucleotide polymorphism (SNP) associated with renal dysfunction after LT using the SNP array for the Japanese population, Japonica Array NEO, comprising a total of 66,883 markers. Three SNPs, rs10277115, rs4690095 and rs792064 were associated with significant differences in the postoperative change of ΔeGFR and the development of renal dysfunction after LT.
Free Research Field	呼吸器外科
Academic Significance and Societal Importance of the Research Achievements	肺移植後5年生存率は他の臓器移植よりも低く国際平均で約60％であり、肺移植の予後を改善するためには慢性期合併症の克服が鍵となる。慢性移植肺機能不全は肺移植後5年で約50％の肺移植患者に発症するが、本研究成果が新たな慢性移植肺機能不全の診断方法となる可能性がある。また、慢性腎臓病は肺移植後10年で約25％の肺移植患者に発症するが、本研究成果により、その発症を予測し個別化医療に応用できる可能性がある。