2022 Fiscal Year Final Research Report
The coagulation abnormality and vascular endothelial injury in a mouse model of heat stroke.
Project/Area Number |
19K09442
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 55060:Emergency medicine-related
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Research Institution | Showa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
大滝 博和 昭和大学, 医学部, 准教授 (20349062)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 熱中症 / 中枢神経傷害 / 小脳 / 大脳皮質 / 中長期 / 急性期 / 遅発性 |
Outline of Final Research Achievements |
Heatstroke, patients exhibit neurological symptoms, suggesting cerebellar damage. We studied the cerebellum after heatstroke in mice heatstroke models. In our study, motor coordination disorder significantly appeared 3 weeks post heatstroke and gradually improved to some extent. Although demyelination was detected at 1 and 3 weeks after heatstroke in the cerebellum. The Purkinje cell numbers significantly decreased at 1-, 3-, and 9-weeks post heatstroke. The intensity of synaptophysin and postsynaptic density-95 temporarily appeared to attenuate at 3 weeks post heatstroke. Motor coordination loss occurred a few weeks after heatstroke and recovered to some extent. Late-onset motor impairment was suggested to be caused by cerebellar dysfunctions that were morphologically assessed by myelin staining and immunostaining of Purkinje cells with pre-and postsynaptic markers. These phenomena were associated with late-onset neurological deficits and recovery after heatstroke.
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Free Research Field |
救急集中治療分野
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Academic Significance and Societal Importance of the Research Achievements |
ヒト熱中症では,集学的治療で急性期を乗り越えても, 数週間後の遅発性の神経傷害から離床が進まないことを経験する. 特に, 高齢者では, 離床の時期にリハビリが進まず, ADLが大幅に低下し, もとの生活に戻れないことを多く経験する. 熱中症後に小脳症状が出現することはよく知られているが, 病態についてはほとんどわかってこなかった. 本研究では, マウス熱中症モデルを用いて, 遅発性神経傷害に小脳のPurkinje細胞の脱落, 脱髄, シナプス傷害がおきていることをはじめて報告した. 今後, 熱中症後の神経炎症を抑える方法を見出すことで, 新たな治療戦略の構築につながると考える.
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