Pathological and molecular analysis of cerebral aneurysms using live imaging

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09479
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Kazuhiko Nozaki 滋賀医科大学, 医学部, 教授 (90252452)
• Co-Investigator(Kenkyū-buntansha): 青木 友浩 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (40633144) ; 椎野 顯彦 滋賀医科大学, 神経難病研究センター, 准教授 (50215935) ; 中村 紳一朗 滋賀医科大学, 動物生命科学研究センター, 准教授 (50307980) ; 辻 敬一 滋賀医科大学, 医学部, 助教 (80838045)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 脳動脈瘤 / 動物モデル / 炎症

Outline of Final Research Achievements

Dynamic characteristic motions of cerebral arterial walls and aneurysmal walls were detected using live imaging in rat aneurysmal models, and mechanical factors which promote aneurysmal rupture were analyzed. On the same aneurysmal walls, histological and molecular analysis was performed using immunohistochemical methods.
The Cerebral Artery in Cynomolgus Monkeys (Macaca fascicularis) was investigated using MR and CT images, and formalin -fixed arterial specimens were collected and anatomical features were analyzed, and 7 monkeys received surgical procedures to induce cerebral aneurysms, and 6 animals showed cerebral aneurysms in 1 and 2 years after intervention.

Free Research Field

脳神経外科

Academic Significance and Societal Importance of the Research Achievements

脳動脈瘤破裂によるくも膜下出血は予後不良で脳動脈瘤の進展破裂機構の解明は不十分で有効な薬物治療もない。現行の外科治療のリスクも考慮すると脳動脈瘤破裂予防を目指した先制医療としての治療介入は未だ不十分である。げっ歯類での血行力学的および分子学的解析結果から得られた候補薬物をサルモデルで検証することで、新たな薬物治療薬の開発に資する成果と思われる。