2021 Fiscal Year Final Research Report
Macrophage in glioblastoma activates receptor tyrosine kinase
Project/Area Number |
19K09529
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56010:Neurosurgery-related
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Research Institution | Yamaguchi University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 膠芽腫 / 腫瘍関連マクロファージ |
Outline of Final Research Achievements |
Using pathological specimens of glioblastoma, immunostaining of CD11b was performed to see what part of the pathological image of glioblastoma was abundant in tumor associated macrophage. At this time, the pathological images were classified into (1) tumor border region, (2) cell colonization region, (3) necrotic region, and (4) vascular hyperplasia region. Brain tumor transplanted mice were made and administered with BLZ945, an inhibitor of colony stimulating factor-1 (CSF-1), which is strongly involved in tumor-related macrophage function and survival, to create a survival curve. A significant prolongation was obtained. Tumor associated macrophages were found to be infiltrated from around blood vessels toward necrotic tissue, and were found to be closely related to necrotic tissue and hypoxia.
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Free Research Field |
脳腫瘍
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Academic Significance and Societal Importance of the Research Achievements |
根治が得られていない悪性脳腫瘍において、腫瘍細胞のみならず、脳腫瘍内に存在する免疫細胞の役割を解析した。免疫細胞が腫瘍を殺すということにはならず、免疫細胞が腫瘍を増殖させる因子を放出することで腫瘍が増大する。そのため、脳腫瘍内からこの免疫細胞を選択的に抽出し、どのような増殖因子を放出するか検討した。それを薬剤で阻害し、腫瘍の増殖を抑えられることも確認した。実際の脳腫瘍標本の中にどの程度、この免疫細胞が混入するか、どのような場所に混入するかでその役割を解析した。
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