The effect of cyclic AMP on the differentiation of articular chondrocytes.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K09630
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Osaka City University
• Principal Investigator: MANAKA Tomoya 大阪市立大学, 大学院医学研究科, 病院講師 (50382122)
• Co-Investigator(Kenkyū-buntansha): 大田 陽一 大阪市立大学, 大学院医学研究科, 講師 (50633484)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 関節軟骨 / 永久軟骨細胞 / サイクリックAMP

Outline of Final Research Achievements

The purpose of this study was to elucidate the effect of 3′, 5′- cyclic adenosine monophosphate (cAMP) signaling on chondrocytes, especially permanent articular chondrocytes, and its preventive effect on articular cartilage degeneration.
Cartilage tissue was harvested from neonatal mice, then isolated to superficial zone chondrocytes and growth plate chondrocytes. Treatment of superficial zone chondrocytes with forskolin; adenylyl cyclase activator significantly increased the expression of articular cartilage markers lubricin/proteoglycan 4 (PRG-4) and PTHrP. The chondrocyte differentiation marker type X collagen was significantly decreased. The regulation of superficial zone chondrocytes by cAMP signaling could be potentially effective against articular cartilage degeneration.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

表層軟骨細胞においてアデニル酸シクラーゼ活性作用により細胞内サイクリックAMP濃度を上昇させる効果のあるforskolin投与により、関節軟骨マーカーのRNA発現量が上昇し、軟骨分化マーカーの発現量は減少した。
本研究の結果から、サイクリックAMPは関節軟骨の表層に存在する永久軟骨細胞分化を制御することが考えられた。サイクリックAMPシグナルによる関節軟骨表層細胞をターゲットとした関節軟骨変性予防効果が期待できるのではないかと考えている。