Study of utilization of MRT technique in assisted reproduction on cancer survivor

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K09772
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Tohoku University
• Principal Investigator: Tachibana Masahito 東北大学, 医学系研究科, 准教授 (30431571)
• Co-Investigator(Kenkyū-buntansha): 志賀 尚美 東北大学, 大学病院, 講師 (20595558) ; 渡邉 善 東北大学, 大学病院, 講師 (40722567)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: がん生殖 / 卵子凍結 / 核移植 / 細胞質移植

Outline of Final Research Achievements

In the mouse model, 1st polar-body (PB) was prone to intolerance to freeze-thaw. Therefore, 1st PB must be transform to meiotic spindle prior to freezing, and we tested two protocols, i.e., the combination of PB removed MII and hPB1T or the combination of aPB1T and MST with isolated freeze-thaw MII spindle. Because aPB1T is novel procedure, we first compared both creation and development efficiencies to hPB1T. Although creation efficiency was superior in aPB1T, developmental competence was comparable to that of hPB1T oocyte. By comparison of both protocols in step-by-step in freeze-thaw procedure, the combination of PB removed MII and hPB1T was more optimal to achieving increased the number of oocytes to be fertilized and the number of blastocysts to be transferred. Compared with common oocyte freezing method, the combination of PB removed MII and hPB1T increased ４１% oocyte availability for fertilization and １７.4% increased blastocyst for transfer.

Free Research Field

生殖生物学、がん生殖

Academic Significance and Societal Importance of the Research Achievements

本法は凍結卵子数に限定されるがんサバイバーの生殖補助医療において、患者の核遺伝子を継承した受精可能な卵子数と移植可能な胚盤胞数の増加に寄与する可能性がしさされ、卵巣機能低下難治性不妊症例の低採卵数を打破する可能性が示唆された。