2022 Fiscal Year Final Research Report

Dynamic Analysis of the Transition from Human Immature Oocytes to Parthenogenetic Embryos and the Association with Individual Aging and Chromosomal Distribution Abnormalities

Research Project

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Project/Area Number	19K09820
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 56040:Obstetrics and gynecology-related
Research Institution	Akita University
Principal Investigator	Shirasawa Hiromitsu 秋田大学, 医学部附属病院, 講師 (60598019)
Co-Investigator(Kenkyū-buntansha)	熊澤由紀代秋田大学, 医学部附属病院, 准教授 (70400504) 佐藤亘秋田大学, 医学部附属病院, 助教 (10726441) 高橋和政秋田大学, 医学部附属病院, 技術系スタッフ (60791910) 安西実武貴秋田大学, 医学部, 技術系補佐員 (70770444)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	体外成熟培養 / 単為発生 / ヒト配偶子 / 着床前胚発育 / タイムラプスモニタリング / 動的挙動 / 第1極体
Outline of Final Research Achievements	For the first time, it has been clarified that in mature oocytes obtained after in vitro maturation culture of human immature oocytes, there are features associated with pre-implantation embryonic development in the duration from the appearance to the disappearance of the pronucleus in the subsequent parthenogenetic stimulation process. This is different from the timing of the appearance and disappearance of the pronucleus after ICSI in general infertility treatment, and it also suggested a connection with the timing of the first polar body expression during the in vitro maturation process, and the time required from the first polar body expression to parthenogenetic stimulation. There have been no reports to date that continuously analyze the dynamic behavior from in vitro maturation to pre-implantation embryonic development in parthenogenesis in human gametes and embryos. The results obtained are beneficial for examining age-related changes in human embryos.
Free Research Field	生殖医学
Academic Significance and Societal Importance of the Research Achievements	ヒト個体加齢による卵子老化において、染色体分配異常の増加は不妊治療における成績低下の大きな理由である。一方で妊孕性温存における卵子凍結は近年需要が増す中、個体加齢に加えたin vitro agingの影響に関しては検討が不十分であった。第1極体放出という核成熟と、細胞質成熟の時間的乖離が、単為発生後の前核の挙動にどう影響するかを検討し、胚発育との関連を評価した初めての研究であり、今後の卵子老化研究の一助となる。