2021 Fiscal Year Final Research Report
Interaction between exosomal miRNA-21 and inflammasome- role in non-healing wounds
Project/Area Number |
19K10010
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56070:Plastic and reconstructive surgery-related
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Research Institution | University of Miyazaki |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | MicroRNA / Exosome / 創傷治癒 / インフラマソーム |
Outline of Final Research Achievements |
The current research project invesitgated the role of exosomal miR-21 in inducing inflammation in macrophages, and the relationship with NLRP3 inflammasome. In vitro experiments employing macrophages and monocytes revealed that miR-21 overexpression results in a shift towards pro-inflammatory M1 phenotypes, with an involvement of NFkappaB and PI3K signal pathways. NFKappaB p65 was involved in establishing a direct relationship between miR-21 and NLRP3 inflammasome activation, by binding to the promoter region of NLRP3 DNA. The exosomes secreted by these cells were enriched with miR-21, and demonstrated a paracrine effect on the surrounding macrophages, creating a pro-inflammatory milieu.
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Free Research Field |
分子細胞生物学
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Academic Significance and Societal Importance of the Research Achievements |
糖尿病性難治性潰瘍は生活の質(QOL)に大きな影響を及ぼすだけでなく、時には死亡の要因となることも知られている。現在、細胞から体液中に分泌されるエクソソームmiRNA (exosomal miRNA)が細胞間情報伝達システムとして働き、炎症反応における役割や種々の疾患への関与が知られる様になってきた。しかし、糖尿病患者における難治性創傷化の要因と考えられる慢性炎症に関する細胞間情報伝達システムとしてのexosomal miRNAの意義は不明である。本研究では、難治性創傷におけるexosomal miRNA-21の役割を検討し、創傷治癒遅延についての新たな知見を得ることである。
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