2021 Fiscal Year Final Research Report
Regenerative medicine of skin reconstruction using artificial dermis and cultured epithelium with dedifferentiated fat cells (DFAT)
| Project/Area Number |
19K10018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56070:Plastic and reconstructive surgery-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三角 浩司 日本大学, 生物資源科学部, 准教授 (00442682)
松本 太郎 日本大学, 医学部, 教授 (50366580)
樫村 勉 日本大学, 医学部, 准教授 (20570740)
仲沢 弘明 日本大学, 医学部, 教授 (60180270)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 脱分化脂肪細胞 / 人工真皮 |
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| Outline of Final Research Achievements |
We previously found that artificial dermis combined with autologous DFAT cells promotes dermis-like tissue construction and angiogenesis in full skin layer defects in rats. Here, we investigated the potential clinical applications of artificial dermis combined with human DFAT cells in immunodeficient SCID mice. We created full-thickness skin defects on the dorsum, then transplanted a standard artificial dermis into it, and divided into two groups, control group and treated with human DFAT group. Tissues were harvested from transplanted areas and histologically tested.Histological findings revealed obviously thicker dermis-like tissues in the treated mice.The numbers of new blood vessels did not appreciably differ after 1 week, but were abundant in the treated mice at 2 weeks. Immunostaining with anti-human CD31 monoclonal antibody was positive at 3 weeks, which suggest that the human DFAT differentiated into endothelial cells.
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| Free Research Field |
皮膚再生医療
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| Academic Significance and Societal Importance of the Research Achievements |
DFATは脂肪組織から単離した成熟脂肪細胞を脱分化させることにより高い増殖能と間葉系幹細胞(MSC)と同等の多分化能を示すいわゆる人工MSCともいえる細胞群である。DFATを用いることの優位性は少量の成熟脂肪組織から純粋な細胞群を短期間に多量に調整することが可能な点である。今回の研究ではヒトDFATを用いた実験により再現性および移植安全性を検証して臨床応用への基礎データとするとともに、DFATの作用機序についても更に詳細な検討を加えた。その結果、ヒトDFATにより先行実験を同等の効果が再現され、また、DFATが血管内皮細胞に分化して血管新生に寄与していることが示された。
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