Development of periodontal tissue regenerative allograft cell therapy by using clumps of MSCs and chondrogenic induction

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Kajiya Mikihito 広島大学, 医系科学研究科(歯), 助教 (00633041)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 間葉系幹細胞集塊 / C-MSCs / 軟骨誘導 / 軟骨内骨化 / 免疫原性

Outline of Final Research Achievements

Previously, we have developed three-dimensional (3D) clumps of MSCs/ECM complexes (C-MSCs), which consisted of cells and self-produced ECM, that can be grafted into bone lesion areas without an artificial scaffold. In this present study, we have generated C-MSCs by using a chondro-inductive medium (CIM-C-MSCs), of which transplantation can induce successful bone regeneration via endochondral ossification. On the other hand, we have also revealed that C-MSCs treated with IFNg (C-MSCs-g) increased immunomodulatory enzyme IDO expression. Xenotransplantation of human C-MSCs-g into rat calvarial defect model attenuated host immune rejection and facilitated bone regeneration. More importantly, this property was retained even after its cryopreservation.

Free Research Field

再生療法

Academic Significance and Societal Importance of the Research Achievements

C-MSCsは人工材料を用いることなく欠損部に移植可能で、組織再生効果を発揮できる。その社会実装のためには、その安全性・有効性を高める必要がある。
本研究成果によって、低栄養状態に強く、軟骨内骨化を発揮できるC-MSCsが樹立できたことはより信頼度の高い骨再生医療となりえる。さらに、移植拒絶を逃れるほど免疫制御能を高めたC-MSCsを凍結保存出来ることは、ドナー細胞から作製したC-MSCsを備蓄し、患者必要時に速やかかつ確実に提供可能な他家骨組織再生療法の実現に繋がる。