Analysis of metabolic adaptation and three-dimensional structure changes caused by regenerative therapy with FGF-2.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10166
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Osaka University
• Principal Investigator: NOZAKI TAKENORI 大阪大学, 歯学部附属病院, 准教授 (30263304)
• Co-Investigator(Kenkyū-buntansha): 石濱 泰 京都大学, 薬学研究科, 教授 (30439244) ; 豊田 岐聡 大阪大学, 理学研究科, 教授 (80283828) ; 三浦 治郎 大阪大学, 歯学部附属病院, 助教 (70437383) ; 清水 真人 大阪大学, 歯学部附属病院, 医員 (70380277)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: FGF-2 / 歯周組織再生 / オミクス解析

Outline of Final Research Achievements

Tissue regeneration was well induced at the test site using FGF-2 compared to the control site. Immunohistochemical analysis showed that multiple factors involved in angiogenesis and tissue regeneration were expressed at both the test site and the control site. It was also suggested that the expression of osteopontin was increased on the test site at 4 weeks after surgery.
Omics analysis suggested that there were differences between the test site and the control site in the transition of the peak pattern of the TIC chromatogram at each time point after surgery.
These results suggest that FGF-2 alters metabolism and cell-cell interactions involved in tissue healing and regulates expression of factors necessary for tissue regeneration effectively.

Free Research Field

歯周治療学

Academic Significance and Societal Importance of the Research Achievements

歯周組織再生療法の効果を最大限に高めるためには、局所の微小環境を治癒・再生に適した状態へと導くことが肝要である。そこで、現在最も有効性の高い歯周組織再生療法薬であるFGF-2を用いた再生治療により、治癒の過程にどのような影響が生じ、治癒の場の微小環境にどのような変化が生じるのかを、オミクスの手法を用いて解析した。その結果、FGF-2を用いることにより、組織の治癒に関与する細胞の代謝や細胞間相互作用に変化が生じて、効果的な治癒・再生が進行し得る微小環境が構築されることにより、良好な組織再生が得られることが示唆された。