Mechanism of stemness regulation of mesenchymal stem cells on spheroid formation

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K10192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Matsumoto Dental University
• Principal Investigator: LI Xianqi 松本歯科大学, 歯学部, 准教授 (60350831)
• Co-Investigator(Kenkyū-buntansha): 各務 秀明 松本歯科大学, 総合歯科医学研究所, 教授 (80242866) ; 溝口 利英 東京歯科大学, 歯学部, 准教授 (90329475)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 間葉系幹細胞 / スフェロイド / stemness / 遺伝子発現 / シグナル

Outline of Final Research Achievements

In this study, we used a specific low-adhesion culture plate to form spontaneous spheroids and investigated the gene expression profile after 24 hours. Spontaneous spheroids showed significantly higher expression of stem cell markers, EGF and IGF1, and the expression of GM-CSF were suppressed compared with those of adherent cells. Those results suggest that the signal transduction relating to cell division was accelerated, while the expression of inflammatory cytokines was suppressed during the spontaneous spheroid formation.

Free Research Field

口腔再生医学

Academic Significance and Societal Importance of the Research Achievements

自発的スフェロイドは、高い幹細胞性を有するとともに、生体に近い細胞－細胞間、細胞－細胞外基質間の相互作用を再現することが可能である。スフェロイド形成過程における遺伝子発現の解析は、幹細胞科学の発展のみならず、、安定したスフェロイド培養やスフェロイドに関する評価法の確立など幅広い再生医療の臨床応用や、新たな医薬品開発のためのツールとしての応用も期待される。