2021 Fiscal Year Final Research Report
Challenge of controling tooth root length by HDAC3 inhibition of dental mesenchym
| Project/Area Number |
19K10220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
江草 宏 東北大学, 歯学研究科, 教授 (30379078)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 間葉系幹細胞 / 歯胚再生 / エピジェネティクス / HDAC3 / 歯根成長制御 |
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| Outline of Final Research Achievements |
The objective of this study is to control tooth root by inhibiting HDAC3 of dental mesenchyme.
Our MSC-spheroids which showed neural crest stem cell like phenotype highly expressed HDAC9, HDAC11 and down regulated ESCO2, AURKA, and AURKB by analyzing PCR array. Our experiments suggested that the epigenetic change of MSC-spheroids was related to the gene expression of cell proliferation and differentiation. In addition, we performed tooth organ germ method by using MSC-spheroid and fetal dental epithelium. We successfully generate tooth organ using our MSC-spheroids. The next step, we will use HDAC inhibitor to organ germ to make clear whether HDAC inhibitor can control tooth root length.
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| Free Research Field |
歯科補綴学、再生歯学
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトの永久歯は中切歯、側切歯から第２大臼歯までと一般的には智歯も含めると32本存在するが、形態や大きさはそれぞれの歯種でほぼ一定である。現在、歯の再生は実現化に至っていないが、器官原基法を応用可能であった場合、歯の形態や大きさ制御は困難な現状にあり、技術構築が必須となる。我々が発見したHDAC3の発現抑制は、歯の内部や歯根の成長に関わる象牙質の成長を抑制する働きがあることが示唆され、今後歯の再生技術が構築された際、任意の大きさに成長させたは歯胚の成長をストップさせる技術へつながる可能性がある。
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