2023 Fiscal Year Final Research Report

Analysis of the Effects of Bacterial Factors on Bone Regeneration Using Mesenchymal Stem Cells Derived from Elderly Donors

Research Project

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Project/Area Number	19K10251
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57050:Prosthodontics-related
Research Institution	Kagoshima University
Principal Investigator	Shimotahira Naohiro 鹿児島大学, 医歯学総合研究科, 客員研究員 (20710007)
Co-Investigator(Kenkyū-buntansha)	西村正宏鹿児島大学, 医歯学域歯学系, 教授 (00294570) 末廣史雄鹿児島大学, 医歯学域鹿児島大学病院, 講師 (40524781) 原田佳枝鹿児島大学, 医歯学域歯学系, 助教 (60432663) 石井正和鹿児島大学, 医歯学域歯学系, 助教 (00456683)
Project Period (FY)	2019-04-01 – 2024-03-31
Keywords	間葉系幹細胞 / 細菌 / 高齢者
Outline of Final Research Achievements	In prosthetic treatments, patients may experience progressive bone resorption over time, making treatment challenging. Mesenchymal stem cells (MSCs) derived from the mandibular or maxillary bone are particularly advantageous as they are easy to harvest and minimally invasive, thereby reducing patient burden. Clinical researches on their application are ongoing. However, in elderly patients, the accumulation of senescent cells is observed, and their MSCs may also be affected by aging. This study compared the proliferative capacity, senescent cell positive rate, osteogenic and adipogenic differentiation abilities, and SASP factor expression of mandibular MSCs and tubular bone MSCs obtained from young and aged male C57BL/6J mice. The results suggested that aged MSCs contained a higher number of senescent cells, exhibited reduced differentiation abilities, and had increased SASP factor expression.
Free Research Field	歯科補綴学分野
Academic Significance and Societal Importance of the Research Achievements	この研究の社会的意義は、老齢患者の補綴治療の効果向上に寄与する点にある。高齢者は補綴治療の主要な対象であり、老化に伴う骨吸収や細胞機能低下が治療の成功に影響を与える。この研究により、老齢者の顎骨MSCの特性が明らかになれば、より効果的な治療法の開発が可能となり、高齢患者のQOL（生活の質）向上が期待される。また、老化細胞の影響を考慮した再生医療の進展も見込まれ、社会全体の医療水準向上に繋がる重要な知見を提供する。さらに、高齢化社会における医療費の削減にも寄与し、持続可能な医療システムの構築に貢献する可能性がある。