2023 Fiscal Year Final Research Report
Application of cell extract derived from dedifferentiated fat cells to a new treatment for facial palsy
| Project/Area Number |
19K10261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57060:Surgical dentistry-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 友里恵 (山田友里恵) 新潟大学, 医歯学系, 助教 (20804537)
瀬尾 憲司 新潟大学, 医歯学系, 教授 (40242440)
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| Project Period (FY) |
2022-11-15 – 2024-03-31
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| Keywords | 脂肪組織由来幹細胞 / 脱分化脂肪細胞 / 細胞抽出物 / 末梢神経再生 |
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| Outline of Final Research Achievements |
Dedifferentiated fat (DFATs) and adipose tissue-derived stem cells (ADSCs) were established from mouse adipose tissue, and DFATs-derived cell extracts (DFATs-CE) and ADSCs-derived cell extracts (ADSCs-CE) were obtained by repetition of the freezing and thawing on both cells. A proliferative effect on Schwann cells and a protrusion elongation effect on dorsal root ganglion (DRG) neurons / PC12D cells were observed when ADSCs-CE was added to the culture medium.However, these effects disappeared when heated ADSCs-CE were added to the medium.
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| Free Research Field |
末梢神経再生
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| Academic Significance and Societal Importance of the Research Achievements |
末梢性顔面神経麻痺は外傷や頭頚部腫瘍の外科的切除後に発生し、表情筋の機能不全に伴い病的共同運動や顔面拘縮など様々な後遺症を生じるため、患者は自然な表情や発話や摂食が困難となり、身体的・精神的な問題となる。自家神経移植は顔面神経の部分欠損に対するゴールドスタンダードとされているが、神経採取部位の感覚障害やparesthesia，採取可能な神経の長さの限界など問題点は多い。これらの現状に対し、低侵襲に樹立可能で、免疫原性・腫瘍原生が低いと考えられるADSCs-CEを用いた顔面神経麻痺への治療法を開発できれば、患者QOL改善に大きく貢献できると考える。これが本研究の意義である。
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