2022 Fiscal Year Final Research Report
A new strategy for mucosal regeneration by primary adult human epithelial progenitor/"stem cells" keratinocyte from oral mucosa
Project/Area Number |
19K10275
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 57060:Surgical dentistry-related
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Research Institution | The Nippon Dental University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
宮澤 敦子 日本歯科大学, 生命歯学部, 助教 (00706997)
古賀 陽子 東京女子医科大学, 医学部, 教授 (10392408)
近津 大地 東京医科大学, 医学部, 主任教授 (30343122)
八重垣 健 日本歯科大学, 生命歯学部, 教授 (40166468)
里見 貴史 日本歯科大学, 生命歯学部, 教授 (70276921)
松野 智宣 日本歯科大学, 生命歯学部, 教授 (80199827)
米山 勇哉 日本歯科大学, 生命歯学部, 助教 (10759799)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 幹細胞 / 再生医療 / 口腔粘膜 / 細胞シート / ケラチノサイト |
Outline of Final Research Achievements |
The purpose of this study was to evaluate the biological activity of ePUKs in vitro. Primary human oral keratinocyte monolayers are cultured using twice the volume of medium without serum and lacking essential fatty acids. Once the cells reach 80% confluence, they begin to float up into the overlying medium and are called "epithelial pop-up keratinocytes (ePUKs)" allowing the cells to be passaged without the use of trypsin. In addition, the pop-pop cells produced by the monolayer of ePUKs were also evaluated. The cell sheets were prepared with ePUKs and evaluated with immunocytofluorescence biomarker staining. The animal experiments could not be performed, even though we were planning to use the ePUKs sheet for the model mouse of palatal full thickness defects.
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Free Research Field |
幹細胞
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Academic Significance and Societal Importance of the Research Achievements |
臨床現場で必要となる質の高い十分量の幹細胞を採取、また生産することは難しい。本研究では口腔粘膜から、より幹細胞に近いヒト口腔粘膜由来のケラチノサイト"epithelial pop-up keratinocytes (ePUKs)" を獲得する事が出来た。一度に大量の幹細胞様ケラチノサイトを生産・採取することができれば将来、臨床現場において非常に有用な再生医療材料となるであろう。またePUKsに多分化能が認められたのなら、ePUKsで作製された細胞シートは口腔粘膜再生のみならず様々な組織の再生に役立つことが期待できる。
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