2022 Fiscal Year Final Research Report

Elucidation of the mechanisms of transition from odontogenic infection to osteomyelitis of the jaw focusing on angiogenesis and lymphangiogenesis

Research Project

PDF

Project/Area Number	19K10331
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57060:Surgical dentistry-related
Research Institution	Kobe University
Principal Investigator	Akashi Masaya 神戸大学, 医学研究科, 教授 (40597168)
Co-Investigator(Kenkyū-buntansha)	木本明神戸大学, 医学部附属病院, 講師 (30597167) 長谷川巧実神戸大学, 医学部附属病院, 助教 (50546497) 筧康正神戸大学, 医学部附属病院, 特命講師 (70772896)
Project Period (FY)	2019-04-01 – 2023-03-31
Keywords	骨髄炎 / 歯性感染症 / 血管新生 / リンパ管新生
Outline of Final Research Achievements	Bacterial infection contributes to pathogenesis of osteonecrosis of the jaws (ONJ). We used a histopathological approach to assess bacterial colonization in the mandibular condyle and elucidate possible paths of bacterial spread towards the mandibular condyle. Macroscopic bone destruction was apparent especially near the medial side of the cortical wall. Bacteria tended to spread posteriorly and through the medial side of the mandibular cortical wall. The potential for bacterial colonization of the mandibular condyle should be considered during treatment of ONJ. We also confirmed the protein possibly associated angiogenesis under the pathological condition, Junctional Cadherin 5 Associated (JCAD), in human tissues. Immunohistochemical analysis of human tissues revealed JCAD localization at the blood endothelial cell-cell junctions. JCAD expression was more evident in microvessels and arteries in areas affected by inflammation. JCAD expression may be affected by pathological conditions.
Free Research Field	口腔外科
Academic Significance and Societal Importance of the Research Achievements	顎骨壊死がどのように顎骨内を進展するかは十分理解されていませんでしたが、本研究により下顎では皮質骨内側に沿って感染が進展している可能性が明らかになり、よって今後感染を確実に摘除するためには同部の感染を適切に評価する必要性があることが判明しました。また、病的環境下における血管新生に関与している分子の発現をヒトの組織において初めて確認し、今後この分子が感染等の病的環境下における血管・リンパ管新生をヒトの組織において検討する際のマーカーとなりうる可能性を見出しました。