2019 Fiscal Year Research-status Report
Development of optimal exercise therapy to prevent diabetes induced multiple organ complications: a focus on VEGF
Project/Area Number |
19K11543
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Research Institution | University of Tsukuba |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
渡部 厚一 筑波大学, 体育系, 准教授 (30447247)
河野 了 筑波大学, 医学医療系, 講師 (90323295)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 糖尿病 / 合併症 / 運動 |
Outline of Annual Research Achievements |
We conducted many rats experiments. We bought the pre-diabetes rat model when compared with a normoglycemic population, which increases the risk of hippocampal memory dysfunction and type 2 diabetes mellitus (T2DM). We investigated whether the progression of spatial memory dysfunction is delayed with four months of exercise intervention since the pre-diabetic stage in an established model of T2DM rat. Memory function, biochemical, and molecular profiles were assessed in the pre-diabetic stage of OLETF rats, a model of T2DM, with LETO rats as genetic control. Then these rats were subjected to mild- or moderate-intensity running training with a treadmill for four months with repetition of same experiments. Significant dysfunction in hippocampal spatial memory was observed in the pre-diabetic stage of OLETF rats, accompanied by downregulated hippocampal MCT2 levels without alteration in hippocampal glycogen level. Four months mild or moderate exercise from pre-diabetes was effective in significant normalization of glycemic parameters with hippocampal molecular normalization through MCT2, glycogen, and BDNF levels. Finally, we observed the considerable improvement of memory dysfunction in early-T2DM rats. A four months exercise training regimen from pre-diabetes either in light- or moderate-intensity can delay the progression of spatial memory dysfunction with hippocampal MCT2-related-lactate-transport improvement of early-T2DM, implying a potential impact of mild exercise on T2DM. This is a preliminary finding, and we are continuing the experiments.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The current research is on the right track both from the contexts of the timeline and the number of research activities scheduled to be done (100% done with target milestone achievement) with the proper management of project budget and funding. Keeping consistency with original research application and planning, in the first year of this project, we already performed many experiments on the pre-diabetes model. We confirmed that impairment of spatial memory dysfunction already started from the early pre-diabetes phase. Here we use the pre-diabetes phase of OLETF rats, which is an established model of type 2 diabetes. We tried to define the exercise intensity, especially for the mild exercise. Furthermore, we are successful in running the pre-diabetic rats with mild exercise intensity for four months and also able to demonstrate that this mild exercise regimen is beneficial to reverse the memory impairment and diabetes-related peripheral symptoms at early diabetes. Next, we plan to use this exercise regimen for other diabetic models and is possible for human subjects, and we did not face any difficulty in accomplishing the first-year plan for this research.
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Strategy for Future Research Activity |
In the 2nd year of this project, we would like to make much progress in a human study that we already started preparation at the end of the first year. This year, we will conduct several mobile health screening camps in rural Bangladesh and make an effort to diagnose diabetes who were no known cases of diabetes. We will screen diabetes by OGTT, in Bangladesh, in a rural setting, it is challenging to measure HBA1c for diabetes screening. And then, we conduct six months mild exercise program in these newly diagnosed diabetes cases and then check the improvement status on peripheral diabetes symptoms. Further, we will focus on VEGF angiogenic signaling cascade and the related apoptosis signaling cascade in the pre-diabetes hippocampus with or without exercise running. Besides, we tried to explore organ dysfunctions in other vital organs in early diabetes and the related effects of exercise.
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Causes of Carryover |
年度の最終盤に、新型コロナウイルスの影響があり、ヒトの抗体検査用試薬キットを購入しなかったため。状況が落ち着き次第、試薬キットを購入する予定である。
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Research Products
(17 results)
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[Journal Article] A short term lifestyle intervention program through daily walking improves circulatory low HDL level in rural Bangladeshi women.2020
Author(s)
Jesmin, S.; Sohael, F.; Rahman, M.A.; Maqbool, A.; Islam, M.M.; Shima, T.; Shimojo, N.; Yamaguchi, N.; Watanabe, K.; Takeda, F.; i Soya, H.
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Journal Title
J Phys Fitness Sports Med
Volume: 9(4)
Pages: 001-010
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Daily walking habit reduces fasting blood sugar level in rural Bangladeshi population in newly diagnosed diabetic subjects2020
Author(s)
Jesmin, S.; Sohael, F.; Yamaguchi, N.; Maqbool, A.; Shima, T.; Rahman, M.A.; Okazaki, O.; Watanabe, K.; Takeda, F.; Soya, H
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Journal Title
J Phys Fitness Sports Med
Volume: -
Pages: -
Peer Reviewed / Open Access
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[Presentation] Comprehensive analysis of emerging risk factors of coronary heart disease in young population in Bangladesh2019
Author(s)
Rahman, M.A.; Jesmin, S.; Sohael, F.; Sultana, S.N.; Islam, M.M.; Shimojo, N.; Yamaguchi, N.; Yashima, Y.; Kawano, S.; Okazaki, O.; Habib, A.K.M.A.
Organizer
European Society of Cardiology
Int'l Joint Research
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[Presentation] Dual endothelin (ET)-A/B receptor antagonist is better than selective ET-A receptor antagonist in ameliorating the decreased VEGF signaling and inadequate coronary collateral development in early Diabetes2019
Author(s)
Jesmin, S.; Rahman, M.A.; Sohael, F.; Sultana, S.N.; Islam, M.M.; Shimojo, N.; Yamaguchi, N.; Yashima, Y.; Kawano, S.; Okazaki, O.; Habib, A.K.M.A.
Organizer
European Society of Cardiology
Int'l Joint Research
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[Presentation] Hypertension among the adult population in Bangladesh: Results from a nation-wide survey2019
Author(s)
Rahman, M.A.; Jesmin, S.; Sohael, F.; Sultana, S.N.; Islam, M.M.; Shimojo, N.; Yamaguchi, N.; Yashima, Y.; Kawano, S.; Okazaki, O.; Habib, A.K.M.A
Organizer
European Society of Cardiology
Int'l Joint Research
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[Presentation] A four months exercise regimen from pre-diabetes is effective in the prevention of hippocampal spatial memory dysfunction in early type 2 diabetes: potential impact on hippocampal downregulated MCT-22019
Author(s)
Jesmin, S.; Rahman, M.A.; Shima, T.; Takahashi, K.; Matsuishi, Y.; Iwashima, Y.; Yamaguchi, N.; Kawano, S.; Shimojo, N.; Okazaki, O.; Soya.H
Organizer
World Congress of European Society of Cardiology
Int'l Joint Research
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[Presentation] Disrupted VEGF angiogenic signaling system in metabolic syndrome subjects in rural Bangladeshi women2019
Author(s)
Jesmin, S.; Rahman, M.A.; Sohael, F.; Sultana, S.N.; Islam, M.M.; Shimojo, N.; Yamaguchi, N.; Yashima, Y.; Kawano, S.; Okazaki, O.; Habib, A.K.M.A.; Watanabe, K
Organizer
World Congress of European Society of Cardiology
Int'l Joint Research
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