Fundamental research on the molecular mechanisms of cellular senescence induced by anisomycin treatment

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K11749
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Iwate Medical University
• Principal Investigator: Ushijima Hironori 岩手医科大学, 薬学部, 助教 (90509043)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: アニソマイシン / 細胞老化 / RNA-seq

Outline of Final Research Achievements

Colon cancer cell lines were cultured in medium containing a pre-determined concentration of anisomycin, miRNAs and mRNAs were prepared from these cells, and microarray and RNA sequencing were performed. The microarray analysis of miRNAs showed that six types of miRNAs including previously reported miRNAs were newly identified. The RNA sequencing analysis suggested that decreased expression of laminin-related genes and NFKB2 and increased expression of tumor suppressor genes such as ATF3 were associated with growth suppression and induction of cellular senescence. By searching with public databases, we were able to identify eight new transcription factors that may regulate these genes expression.

Free Research Field

薬学、分子生物学、生化学

Academic Significance and Societal Importance of the Research Achievements

本研究で見出されたLaminin関連遺伝子、NFKB2、およびATF3などの遺伝子は、癌細胞において細胞老化を誘導する際のターゲットとして創薬分野での応用が期待できる。また、老化を検出する際の新しいマーカーとして使用できる可能性がある。これらの遺伝子の転写因子は、老化シグナル（老化ストレス）に対する初期センサーとして機能している可能性があり、その代謝回転の研究が進むことで癌細胞における老化誘導を効率的に行える可能性がある。