Anti-inflammatory effects of delta-tocopherol on mouse adipocytes and adipose tissues

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K11753
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 59040:Nutrition science and health science-related
• Research Institution: Kanagawa Institute of Technology
• Principal Investigator: Kiyose Chikako 神奈川工科大学, 健康医療科学部, 教授 (50272745)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ビタミンE / δ-トコフェロール / 脂肪組織 / 抗炎症作用 / 抗肥満

Outline of Final Research Achievements

The study aim was to evaluate the anti-inflammatory effect of vitamin E analogs on mice adipocytes and rat adipose tissues induced inflammation. Consequently, it was found that α-tocopherol inhibited increased gene expression of the inflammatory cytokines, IL-1β,IL-6, and iNOS in co-cultured 3T3-L1 cells and RAW264.7 cells. Subsequently, I used male SD strain rats, which were divided into four groups: high-fat and high-sucrose diet (C), high-fat and high-sucrose diet +LPS intraperitoneal administration (LPS), LPS+α-tocopherol (800mg/kg diet), and LPS+δ-tocopherol (800mg/kg diet) groups. The rats were fed for 4 weeks. After 4 weeks, each rat was administered LPS (1mg/kg body weight) intraperitoneally. Consequently, it was found that δ-tocopherol tended to inhibit increased gene expression of IL-1βin perirenal adipose tissues of rats. Therefore, these results suggest that δ-tocopherol has an anti-inflammatory effect on adipose tissues induced inflammation by LPS.

Free Research Field

食品機能学、分子栄養学

Academic Significance and Societal Importance of the Research Achievements

日本人において罹患率が右肩上がりの生活習慣病の発症を抑制する事は非常に重大な課題である。その発症には肥満から来る肥大化した脂肪組織が炎症誘導される事が明らかになっており、その炎症誘導を抑制する事が生活習慣病の発症予防には重要である。ビタミンEはすでにサプリメントとして広く利用されているが、δ-トコフェロールのサプリメントはほとんどなく、さらに抗炎症作用の効果をうたったサプリメントはない。今回、in vivoの途中までδ-トコフェロールの効果を論証できたので、さらに検討を続ける事で抗炎症効果をもつビタミンEサプリメントが開発できる可能性が示唆された。