Radiation sensitivity of neural stem cells and immature neurons in the fetal mouse striatum.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K12324
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 63020:Radiation influence-related
• Research Institution: Osaka Prefecture University
• Principal Investigator: SHIRAISHI Kazunori 大阪府立大学, 理学(系)研究科(研究院), 助教 (40347513)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 神経幹細胞 / 放射線感受性

Outline of Final Research Achievements

To clarify the radio sensitivity of fetal striatum, DNA repair kinetics and apoptotic activity were evaluated. The results showed that the DNA repair activities of neural stem cells and neurons were comparable, and that DNA repair was terminated by 6 hours post-irradiation. In the region containing neural stem cells, Caspase3-activated cells appeared at 3 hours after irradiation, and in the region containing neurons, Caspase3-activated cells appeared at 8 hours after irradiation. This delayed caspase activation was seen in neurons and triggered cell death. The results suggested that the differential regulation of apoptosis-related genes in the developing central nervous system are involved in the fate of the cells after irradiation.

Free Research Field

放射線生物学

Academic Significance and Societal Importance of the Research Achievements

今回の報告で、胎児線条体における幹細胞と神経細胞はDNA2本鎖切断損傷に対して、同等の修復活性を持つことが示された。その一方で、細胞死を指標とした放射線感受性は異なることが示された。この感受性の違いは、アポトーシス関連遺伝子であるcaspase-3の活性化の関与によるものであることが示唆された。胎内被ばくにおける、神経幹細胞と新生ニューロンの感受性を明らかにすることは、放射線の潜在的リスクを考える上で重要な知見となる。